The lab used to be a fun place where u can say out loud proudly "I also fail to get results ma. Never mind one la, cincai do enough, report can simply explain and lie de..."
But things change when u r doing final year project. When u get positive results, tis is very lucky. When u get negative result, tis is still very normal. The saddest case is when u fail to get any results. Then ur life will be interesting enough, have to find ways to solve and face the pressure from supervisor and limited resources.
So, here are some pictures taken when we're so boring..haha
part of the samples needed to be extracted
sucking blood
some fail to suck properly so blood full of bubbles
the brand new thing in utar, EZ-vision 3 in 1. Can straightly view gel without staining and destaining. no more the blue loading dye
all time cleaner to separate PCI and bloody tips
tis is wat happen when u have limited resources. Air-dry the gloves to be wear again
caught! the thief in autoclave room
like i told u, the lab is very boring...
some ppl just won't miss any chances to bully ppl with tis slavery contract
some ppl even help to deco the boring lab
no need to caution, not hot at all, keke
this is full of failure-rate extraction
when things start better a bit, the DNA bands start to smile to u instead of linear band (actually DNA smiling is not a good image, hehe)
wanna take pictures in the lab but scare the camera "infected", so sterilize the camera with ethanol spray, swt
5 comments:
There is one more thing worse than not getting any results: your negative control got bands! It is worse if you are doing PCR since there so many reagents to test out.
All 4 of us in kampar faced this problem. Worst of all, the contamination is not due to ourselves, but previous cross contamination. It took zhi xian and I 3 weeks to find out the culprit (which is the Taq, I will tell you the whole story when we get back to setapak, hehe) using different experiments. For HWY, he is not that lucky. He still stuck at identifying what are the contaminants.
luckily my negative control tak da band, but the sad case is positive control also tak da band....and the pcr product band is too low intensity...now have to try to increase the number of cycles in pcr see can get clearer band bo...sad
then good luck lor... maybe decrease your annealling temp could help. too high might be too specific and stringent. try lower 5 degrees. I know setapak has 1 gradient pcr. Use it to try different temp for anneal. i have a similar case also. lowered the temp and got the bands. Or maybe can increase DNA template/Taq. Cycles might help also.
still got time to take pictures huh?hahahaha...
shud enjoy the process even though it is tough =p...
good luck and add oil la...^^
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